Overproduction of homocysteine exerts toxic effect on endothelial linage of blood vessels supplying blood into the brain and developing condition of Stroke. Methylenetetrahydrofolatereductase (MTHFR) plays a central role in regulation of homocysteine concentration in the cell. The (677C>T) polymorphic variant of MTHFR gene is known to hyperhomocysteinemia and hens 677C<T MTHFR variant may be a factor for stroke susceptibility. This study investigated whether the genetic variability in the MTHFR gene is related with overproduction of homocysteine as well as susceptibility for Stroke in the central Indian population. A total of 100 medically certified Stroke patients (case) and 223 healthy subjects (control) were recruited from central India as a sample for this investigation. We found a significant (P<0.0001) deference in mean values of tHyc between case and control. ‘T’ allele was present in higher proportion in Stroke patients (P=0.06) as compared with Healthy control group. We concluded that the homozygous MTHFR 677T condition made moderate susceptibility for hyperhomocysteinemia and Stroke.